Cyclic negative pressure promotes chondrocyte growth: Association of IGF-2 with EGR-1

Authors

  • Xiaoyu Li School of Medicine, Jianghan University, Wuhan, China https://orcid.org/0009-0006-6680-0539
  • Lixia Huang Tianyuan Translational Medicine Research and Development Team, School of Medicine, Jianghan University, Wuhan, China; Institute of Translational Medicine, Wuhan College of Arts and Science, Wuhan, China
  • Bingxue Liu School of Medicine, Jianghan University, Wuhan, China
  • Zehua Zhang School of Medicine, Jianghan University, Wuhan, China
  • Guoyong Zhou Tianyuan Translational Medicine Research and Development Team, School of Medicine, Jianghan University, Wuhan, China
  • Zirui Guo Department of Materials, Swiss Federal Institute of Technology, Zurich, Switzerland
  • Jiuhong Song Wuhan FL Medical Science and Technology Ltd., Wuhan, China
  • Xiang Wang School of Medicine, Jianghan University, Wuhan, China; Tianyuan Translational Medicine Research and Development Team, School of Medicine, Jianghan University, Wuhan, China

DOI:

https://doi.org/10.17305/bb.2024.10487

Keywords:

Osteoarthritis, chondrocytes, biomechanics, cell proliferation, EGR-1, IGF-II

Abstract

Knee osteoarthritis (KOA) is one of the most common degenerative joint diseases in the elderly worldwide. The primary lesion in patients with KOA is the degeneration of articular cartilage. This study aimed to observe the biological effects of cyclic negative pressure on C28/I2 chondrocytes and to elucidate the underlying molecular mechanisms. We designed a bi-directional intelligent micro-pressure control device for cyclic negative pressure intervention on C28/I2 chondrocytes. Chondrocyte vitality and proliferation were assessed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays. The extracellular matrix was analyzed using real-time fluorescence quantitative polymerase chain reaction (PCR) and western blot, while the molecular mechanism of the chondrocyte response to cyclic negative pressure was explored through mRNA sequencing. Experimental data demonstrated that cyclic negative pressure promoted chondrocyte proliferation and upregulated the expression of chondrocyte-specific protein, namely the collagen type II alpha 1 chain (COL2A1) protein, and the transcription factor SRY-box transcription factor 9 (SOX9). Additionally, RNA sequencing analysis revealed that the gene levels of insulin-like growth factor 2 (IGF-2) and early growth response 1 (EGR-1) were significantly elevated in the cyclic negative pressure group. This study demonstrates that cyclic negative pressure stimulates the proliferation of C28/I2 chondrocytes by promoting the expression of EGR-1 and IGF-2. This new discovery may provide novel insights into cartilage health and KOA prevention. 

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Cyclic negative pressure promotes chondrocyte growth: Association of IGF-II with EGR-1

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Published

17-10-2024

Data Availability Statement

Data are contained within the article and supplementary materials. RNA-seq data will be shared by the corresponding authors upon reasonable request.

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Research article

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How to Cite

1.
Cyclic negative pressure promotes chondrocyte growth: Association of IGF-2 with EGR-1. Biomol Biomed [Internet]. 2024 Oct. 17 [cited 2024 Dec. 4];24(6):1761–1775. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/10487