Long-term smoking contributes to aging frailty and inflammatory response

Authors

  • Huijin Hou China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China; Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
  • Yidi Chai China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China
  • Ting Zhang China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China
  • Yue Liang China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China; Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
  • Lan Huang China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China; Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
  • Xu Cao China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China; Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
  • Shufang Liang China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China; Harmful Components and Tar Reduction in Cigarette Key Laboratory of Sichuan Province, Chengdu, China; Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China

DOI:

https://doi.org/10.17305/bb.2024.11722

Keywords:

Smoking, frailty, tumor necrosis factor- α, TNF-α, interleukin-1 β, IL-1β, inflammation

Abstract

In recent years, the health challenges linked to frailty in the elderly, particularly those worsened by cigarette smoke, have become more pronounced. However, quantitative studies examining the impact of smoking dosage on frailty in this population remain limited. To address this gap, we developed a model using smoke-exposed elderly mice. Fifteen-month-old C57BL/6J mice were exposed to smoke from two burning cigarettes for 15 min in a whole-body chamber. This exposure occurred 4, 6, and 8 times daily for 30 days, representing low, medium, and high smoking dosages, respectively. Frailty levels were assessed through rotation and grip strength tests, alongside lung histopathology and inflammatory factor protein expression analyses across the three dosage groups. Additionally, we used the Gene Expression Omnibus (GEO) database to validate the correlation between frailty and inflammation in elderly smokers, facilitating cross-comparisons between animal model findings and human sample data. Our results show that mice exposed to high-dose smoking were significantly more prone to frailty, with notable reductions in maximal grip strength (P < 0.01) and drop time (P < 0.001). Among human samples, 69.2% of elderly smokers exhibited a frailty phenotype, compared to just 15.4% of nonsmokers. Both smoking-exposed mice and elderly smokers demonstrated upregulation of tumor necrosis factor-α (TNF-α) and interleukin-1 β (IL-1β) in lung tissue and serum. Mechanistically, this upregulation activates the NF-κB signaling pathway. Our findings quantitatively link smoking-induced frailty to increased levels of TNF-α and IL-1β, providing experimental evidence for the diagnosis and prevention of frailty in elderly populations.

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Long-term smoking contributes to aging frailty and inflammatory response

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Published

17-01-2025

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Research article

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How to Cite

1.
Long-term smoking contributes to aging frailty and inflammatory response. Biomol Biomed [Internet]. 2025 Jan. 17 [cited 2025 Feb. 12];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/11722