TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro

Yanli Ge; Junjie Zhang; Jianchun Cao; Qiong Wu; Longe Sun; Likun Guo; Zhirong Wang

doi:10.17305/bjbms.2012.2499

Authors

Yanli Ge Department of Gastroenterology, Tongji Hospital, Tongji University
Junjie Zhang Department of Gastroenterology, Tongji Hospital, Tongji University
Jianchun Cao Department of Gastroenterology, Tongji Hospital, Tongji University
Qiong Wu Department of Gastroenterology, Tongji Hospital, Tongji University
Longe Sun Department of Gastroenterology, Tongji Hospital, Tongji University
Likun Guo Department of Gastroenterology, Tongji Hospital, Tongji University
Zhirong Wang Department of Gastroenterology, Tongji Hospital, Tongji University

DOI:

https://doi.org/10.17305/bjbms.2012.2499

Keywords:

Trefoil Factor Family 1, gastric cancer, apoptosis, in vitro

Abstract

Trefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC.

The TFF1 expression in different gastric mucosas was measured with immunohistochemistry. Then, siRNA targeting TFF1 or plasmids expressing TFF1 gene were transfected into BGC823 cells, SGC7901 cells and GES-1 cells. The cell proliferation was detected with MTT assay and apoptosis and cell cycle measured by flow cytometry.

From normal gastric mucosa to mucosa with dysplasia and to gastric cancer, the TFF1 expression had a decreasing trend. Down-regulation of TFF1 expression significantly reduced the apoptosis of three cell lines and markedly facilitated their proliferation but had no significant effect on cell cycle. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle. TFF1 can inhibit proliferation and induce apoptosis of GC cells in vitro.