In Vitro Research of the Alteration of Neurons in Vagal Core in Medulla Oblongata at Asphyxic Deaths

  • Naim Haliti Department of Forensic Medicine, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Hilmi Islami Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Nevzat Elezi Department of Microbiology, Faculty of Medicine, University of Tetovo
  • Ragip Shabani Department of Pathology, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Bedri Abdullahu Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Gani Dragusha Department of Cardiology, Faculty of Medicine, University of Prishtina, Clinical Centre
Keywords: death, strangulation, asphyxia, nuclei, vagus, stereometry

Abstract

The aim of this study was to research the morphological changes of neurons in the vagus nerve nuclei in medulla oblongata in asphyxia related death cases. Morphological changes that were investigated were mainly in the dorsal motor respiratory center (DMRC), nucleus tractus solitarius (nTS) and nucleus ambigus (nA) in the medulla oblongata. In our research, the autopsy material from asphyxia related death cases was used from various etiologies: monoxide carbon (CO), liquid drowning, strangulation, electricity, clinical-pathological death, firing weapon, explosive weapon, sharp and blunt objects and death cases due to accident. The material selected for research was taken from medulla oblongata and lungs from all lobes. The material from the medulla oblongata and lungs was fixed in a 10% solution of buffered formalin. Special histochemical methods for central nervous system (CNS) were employed like: Cresyl echt violet, toluidin blue, Sevier-Munger modification and Grimelius. For stereometrical analysis of the quantitative density of the neurons the universal testing system Weibel M42 was used. The acquired results show that in sudden asphyxia related death cases, there are alterations in the nuclei of vagal nerve in form of: central chromatolysis, axonal retraction, axonal fragmentation, intranuclear vacuolization, cytoplasmic vacuolization, edema, condensation and dispersion of substance of Nissl, proliferation of oligodendrocytes, astrocytes and microglia. The altered population of vagus nerve neurons does not show an important statistica! significarne compared to the overall quantity of the neurons in the nuclei of the vagus nerve (p<0,05).

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Published
2010-08-20
How to Cite
1.
Haliti N, Islami H, Elezi N, Shabani R, Abdullahu B, Dragusha G. In Vitro Research of the Alteration of Neurons in Vagal Core in Medulla Oblongata at Asphyxic Deaths. Bosn J of Basic Med Sci [Internet]. 2010Aug.20 [cited 2019May20];10(3):251-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2695
Section
Short Communication

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