In Vitro Examination of Degenerative Evolution of Adrenergic Nerve Endings in Pulmonary Inflamatory Processes in Newborns

  • Hilmi Islami Department of Pharmacology, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Ragip Shabani Department of Patology, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Naim Haliti Department of Forensic Medicine, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Sadi Bexheti Department of Anatomy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Rozafa Koliqi Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Denis Raka Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Aziz Sukalo Drugs factory-Bosnalijek -Sarajevo, Bosnalijek dd
  • Rusmi Izairi Department of Pharmacy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Hilmi Dauti Department of Anatomy, Faculty of Medicine, University of Prishtina, Clinical Centre
  • Nazim Qehaja Department of Patology, Faculty of Medicine, University of Prishtina, Clinical Centre
Keywords: adrenergic receptors, human trachea, bronchus and pulmonary tissue

Abstract

Morphological aspect of tracheal preparations and pulmonary tissue was studied in vitro. The material was obtained from autopsy of newborns that died from different causes. Examinations were made in different gestational periods (immature 23-29 weeks; premature 30-37 weeks; mature >38 weeks). Material for examination was obtained up to 6 hours after death. Pulmonary and tracheal tissue was incubated for fixation in buffered formalin (10%). Special histochemical and histoenzymatic methods were used for coloring of pulmonary and tracheal tissue and the activity of ATP-ase and dopaoxidase was monitored. Cut out models were made in series of 7μ, 10 μ and 20 μ. In peripheral axons of tracheobronchial pathways, degenerative alterations of adrenergic nerve endings in lung inflammatory processes were documented. These morphologic neuronal changes were described: Walerians degeneration, neuro-axonal degeneration and segment demyelinisation. These changes are well seen with argentafine coloring (Sevier-Munger modification for nerve endings) and with dopaoxidase reaction. In mature newborns that died from respiratory distress syndrome, we found different forms of metabolic and toxic degenerative damage in peripheral axons, such as: segment demyelinisation, neurotubular fragmentation, Schwan cell proliferation, fragmentation and bulging out of axonal neurotubules and neurofilaments. In tracheo-bronchial tissue, chromafine granules are homogenously distributed on Lamina propria layer and through glandular structures. This gives as a contradiction, according to some authors, that adrenergic nerve fibers for muscle tissue are absent and that adrenaline and noradrenalin diffuse in muscle tissue from interstice.

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Published
2008-08-20
How to Cite
1.
Islami H, Shabani R, Haliti N, Bexheti S, Koliqi R, Raka D, Sukalo A, Izairi R, Dauti H, Qehaja N. In Vitro Examination of Degenerative Evolution of Adrenergic Nerve Endings in Pulmonary Inflamatory Processes in Newborns. Bosn J of Basic Med Sci [Internet]. 2008Aug.20 [cited 2019Apr.18];8(3):220-5. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2921
Section
Pathology

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