Effect of clozapine on locomotor activity and anxiety-related behavior in the neonatal mice administered MK-801


  • Neslihan Pınar Faculty of Medicine, Medical Pharmacology, Mustafa Kemal University, Hatay, Turkey
  • Kubra Akillioglu Faculty of Medicine, Medical Physiology, Cukurova University, Adana, Turkey
  • Fatih Sefil Faculty of Medicine, Medical Physiology, Mustafa Kemal University, Hatay
  • Harun Alp Faculty of Medicine, Medical Pharmacology, Mustafa Kemal University, Hatay, Turkey
  • Mustafa Sagir Faculty of Medicine, Medical Pharmacology, Gaziosmanpasa University, Tokat
  • Ahmet Acet Faculty of Medicine, Medical Pharmacology, Inonu University, Malatya, Turkey




MK-801, Clozapine, Open Field, Elevated Plus Maze, Neonatal mice


Atypical antipsychotics have been used to treat fear and anxiety disturbance that are highly common in schizophrenic patients. It is suggested that disruptions of N-methyl-d-aspartate (NMDA)-mediated transmission of glutamate may underlie the pathophysiology of schizophrenia. The present study was conducted to analyze the effectiveness of clozapine on the anxiety-related behavior and locomotor function of the adult brain, which had previously undergone NMDA receptor blockade during a developmental period. In order to block the NMDA receptor, male mice were administered 0.25 mg/kg of MK-801 on days 7 to 10 postnatal. In adulthood, they were administered intraperitoneally 0.5 mg/kg of clozapine and tested with open-field and elevated plus maze test, to assess their emotional behavior and locomotor activity. In the group receiving MK-801 in the early developmental period the elevated plus maze test revealed a reduction in the anxiety-related behavior (p<0.05), while the open-field test indicated a decrease in locomotor activity (p<0.01). Despite these reductions, clozapine could not reverse the NMDA receptor blockade. Also, as an atypical antipsychotic agent, clozapine could not reverse impairment in the locomotor activity and anxiety-related behavior, induced by administration of the MK-801 in neonatal period.


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Effect of clozapine on locomotor activity and anxiety-related behavior in the neonatal mice administered MK-801


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Effect of clozapine on locomotor activity and anxiety-related behavior in the neonatal mice administered MK-801. Biomol Biomed [Internet]. 2015 Aug. 11 [cited 2024 Jun. 20];15(3):74-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/472