LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma via down-regulating Wnt/β-catenin signaling pathway

  • Chunhui Zhou Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
  • Hulin Zhao Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
  • Shuiwei Wang Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
  • Chao Dong Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
  • Fan Yang Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
  • Jianning Zhang Department of Neurosurgery, Medical School of Chinese PLA General Hospital, Beijing, China
Keywords: lncRNA, ADAMTS9-AS1, glioma, prognosis, Wnt/β-catenin

Abstract

The long non‐coding RNA antisense 1 ADAMTS9-AS1 has been reported to serve as an oncogene or tumor suppressor in several tumors, including colorectal cancer and hepatocellular carcinoma. Nevertheless, the clinical significance and biological behaviors of ADAMTS9-AS1 in glioma still remain unclear. Therefore, the goal of this study was to evaluate the functional roles and potential mechanisms of ADAMTS9-AS1 in glioma cells. Using quantitative real-time PCR analysis, we found that ADAMTS9-AS1 was upregulated in glioma tissues and cells in comparison to corresponding controls. ADAMTS9-AS1 expression level was correlated to tumor size (p=0.005) and WHO grade (p=0.002). Kaplan-Meier analysis and Cox multivariate analysis showed that ADAMTS9-AS1 could serve as an independent prognostic factor affecting the overall survival of glioma patients. Functionally, depletion of ADAMTS9-AS1 significantly suppressed the proliferation, migration and invasion in glioma cell lines (U251 and U87), as shown via CCK-8 assay, Edu corporation assay, wound healing assay and transwell assay. Furthermore, we demonstrated that knockdown of ADAMTS9-AS1 suppressed Wnt1, β-catenin, c-myc and PCNA, while upregulating E-cadherin expression. In conclusion, our data revealed that ADAMTS9-AS1 confers oncogenic function in the progression of glioma, thus targeting ADAMTS9-AS1 might be a promising therapeutic strategy for this disease.

Downloads

Download data is not yet available.
LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma via down-regulating Wnt/β-catenin signaling pathway
Published
2021-12-11
How to Cite
1.
Zhou C, Zhao H, Wang S, Dong C, Yang F, Zhang J. LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma via down-regulating Wnt/β-catenin signaling pathway. Bosn J of Basic Med Sci [Internet]. 2021Dec.11 [cited 2022Jan.18];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/6199
Section
Molecular Biology