Polymorphisms in DNA repair genes XRCC2 and XRCC3 risk of gastric cancer in Turkey

Authors

  • İlhami Gok Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, 36100 Kars, Turkey
  • Meryem Baday Departments of Biology, Faculty of Science and Literature, Kafkas University , Kars, Turkey
  • Suleyman Cetinkunar Departments of Surgery , Adana Numune Training and Research Hospital, Adana , Turkey
  • Kemal Kilic Departments of Surgery, Kartal Training and Research Hospital ,İstanbul , Turkey
  • Bulent Caglar Bilgin Department of Surgery , School of Medicine , Kafkas University, Kars , Turkey

DOI:

https://doi.org/10.17305/bjbms.2014.4.7

Keywords:

gastric cancer, XRCC2 and XRCC3 genes, polymorphism, Turkey

Abstract

We studied the prevalence of polymorphisms in genes XRCC2 and XRCC3 in stomach cancer patients who lived in North Eastern Turkey. A total of 61 cancer patients and 78 controls were included in this study. Single nucleotide changes were studied in XRCC2 and XRCC3 genes at locus Arg188His and Thr241Met. Blood samples were taken from the patients and controls, and DNA was isolated. The regions of interest were amplified using a polymerase chain reaction method. After amplification, we used restriction enzymes (HphI and NcoI) to digest the amplified product. The digested product was then run through gel electrophoresis. We identified changes in the nucleotides in these specific regions. It was found that the Arg188His polymorphism of the XRCC2 gene was about 39% (24 out of the 61) among cancer patients. However, only 15% (12 out of 78) of the control group indicated this polymorphism. We also observed that 18 of the 61 cancer patients (29%) carried the Thr241Met polymorphism of the XRCC3 gene whereas 11 of the 78 (14%) individuals in the control group had the polymorphism. Our results showed a significant difference in polymorphism ratios between the cancer patients and healthy control group for the regions of interest. This result clearly showed that these polymorphisms increase the risk of stomach cancer and might be a strong marker for early diagnosis of gastric cancer.

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Author Biographies

  • İlhami Gok, Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, 36100 Kars, Turkey

    Human genetics, Molecular Biology , Bioengineering

  • Meryem Baday, Departments of Biology, Faculty of Science and Literature, Kafkas University , Kars, Turkey

    Molecular Biology, genetics

  • Suleyman Cetinkunar, Departments of Surgery , Adana Numune Training and Research Hospital, Adana , Turkey
    Oncology, Surgery
  • Kemal Kilic, Departments of Surgery, Kartal Training and Research Hospital ,İstanbul , Turkey
    Surgery, oncology
  • Bulent Caglar Bilgin, Department of Surgery , School of Medicine , Kafkas University, Kars , Turkey
    general surgery
Polymorphisms in DNA repair genes XRCC2 and XRCC3 risk of gastric cancer in Turkey

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Published

13-09-2014

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Section

Molecular Biology

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How to Cite

1.
Polymorphisms in DNA repair genes XRCC2 and XRCC3 risk of gastric cancer in Turkey. Biomol Biomed [Internet]. 2014 Sep. 13 [cited 2024 Jul. 21];14(4):214-8. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7