Effects of single-dose neuropeptide Y on levels of hippocampal BDNF, MDA, GSH, and NO in a rat model of pentylenetetrazole-induced epileptic seizure

  • Hale Maral Kir Department of Biochemistry, School of Medicine, Kocaeli University
  • Deniz Şahin Department of Physiology, School of Medicine, Kocaeli University
  • Berrin Öztaş Department of Physiology, School of Medicine, Kocaeli University
  • Mert Musul Department of Physiology, School of Medicine, Kocaeli University
  • Sevinc Kuskay Department of Biochemistry, School of Medicine, Kocaeli University
Keywords: epileptic seizure, neuropeptide Y, brain-derived neurotrophic factor, oxidative stress, nitrosative stress

Abstract

Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen and nitrogen species. Th e objective of this study was to investigate the eff ects of Neuropeptide Y on oxidative and nitrosative balance and brain-derived neurotrophic factor levels induced by pentylenetetrazole (a standard convulsant drug) in the hippocampus of Wistar rats. Th ree groups of seven rats were treated intraperitoneally as follows: group  (saline + saline)  ml saline, group  (salin + Pentylenetetrazole)  ml saline  min before Pentylenetetrazole; and group  (Neuropeptide Y + Pentylenetetrazole)  μg/kg Neuropeptide Y  min before  mg/kg Pentylenetetrazole. After  h, the animals were euthanized by decapitation. Hippocampus were isolated to evaluate the malondialdehyde, glutathione, nitric oxide, and brain-derived neurotrophic factor levels in three rat groups. Th e results of this study demonstrated that while intraperitoneally administered neuropeptide Y did not result in a statistically signifi cant diff erence in BDNF levels, its administration caused a statistically signifi cant decrease in malondialdehyde and nitric oxide levels and an increase in glutathione levels in rats with pentylenetetrazole-induced epileptic seizure. Neuropeptide Y were able to reduce nitroxidative damage induced by pentylenetetrazole in the hippocampus of Wistar rats.

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Published
2013-11-20
How to Cite
1.
Maral Kir H, Şahin D, Öztaş B, Musul M, Kuskay S. Effects of single-dose neuropeptide Y on levels of hippocampal BDNF, MDA, GSH, and NO in a rat model of pentylenetetrazole-induced epileptic seizure. Bosn J of Basic Med Sci [Internet]. 2013Nov.20 [cited 2019Nov.18];13(4):242-7. Available from: http://www.bjbms.org/ojs/index.php/bjbms/article/view/2332
Section
Biochemistry