Leukotriene B4 receptor knockdown affects PI3K/AKT/mTOR signaling and apoptotic responses in colorectal cancer

Authors

  • Cui Tang Department of Radiology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China https://orcid.org/0000-0002-7018-5763
  • Aili Wang Center for Clinical Research and Translational Medicine, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China
  • YanLin Zhao Department of Radiology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China https://orcid.org/0000-0002-2089-8028
  • WenYing Mou Department of Radiology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China
  • Jun Jiang Endoscopy Center, Minhang District Central Hospital of Fudan University, Shanghai, China https://orcid.org/0009-0000-3404-2071
  • Jie Kuang Department of Radiology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China
  • Bin Sun Center for Clinical Research and Translational Medicine, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China https://orcid.org/0000-0002-5082-3326
  • Erjiang Tang Center for Clinical Research and Translational Medicine, Yangpu Hospital, School of Medicine, Tongji University

DOI:

https://doi.org/10.17305/bb.2024.10119

Keywords:

Leukotriene B4 receptor (LTB4R), colorectal cancer (CRC), phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway, apoptosis

Abstract

Colorectal cancer (CRC) presents a landscape of intricate molecular dynamics. In this study, we focused on the role of the leukotriene B4 receptor (LTB4R) in CRC, exploring its significance in the disease's progression and potential therapeutic approaches. Using bioinformatics analysis of the GSE164191 and the Cancer Genome Atlas-colorectal adenocarcinoma (TCGA-COAD) datasets, we identified LTB4R as a hub gene influencing CRC prognosis. Subsequently, we examined the relationship between LTB4R expression, apoptosis, and the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway through cellular and mice experiments. Our findings revealed that LTB4R is highly expressed in CRC samples and is pivotal for determining prognosis. In vitro experiments demonstrated that silencing LTB4R significantly impeded CRC cell viability, migration, invasion, and colony formation. Correspondingly, in vivo tests indicated that LTB4R knockdown led to markedly slower tumor growth in mice models. Further in-depth investigation revealed that LTB4R knockdown significantly amplified the apoptosis in CRC cells and upregulated the expression of apoptosis-related proteins, such as caspase-3 and caspase-9, while diminishing p53 expression. Interestingly, silencing LTB4R also resulted in a significant downregulation of the PI3K/AKT/mTOR signaling pathway. Moreover, pretreatment with the PI3K activator 740Y-P only partially attenuated the effects of LTB4R knockdown on CRC cell behavior, emphasizing LTB4R's dominant influence in CRC cell dynamics and signaling pathways. LTB4R stands out as a critical factor in CRC progression, profoundly affecting cellular behavior, apoptotic responses, and the PI3K/AKT/mTOR signaling pathway. These findings not only shed light on LTB4R's role in CRC but also establish it as a potential diagnostic biomarker and a promising target for therapeutic intervention.

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Leukotriene B4 receptor knockdown affects PI3K/AKT/mTOR signaling and apoptotic responses in colorectal cancer

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Published

20-01-2024

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Section

Translational and Clinical Research

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How to Cite

1.
Leukotriene B4 receptor knockdown affects PI3K/AKT/mTOR signaling and apoptotic responses in colorectal cancer. Biomol Biomed [Internet]. 2024 Jan. 20 [cited 2024 May 27];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/10119