Expression and Clinical Significance of P120 Catenin mRNA and Protein in Pancreatic Carcinoma

  • Yang Fei Department of General Surgery, the 81st Hospital of P.L.A., P.L.A. Cancer Center
  • Zhangjun Cheng Department of General Surgery, the Affiliated Zhongda Hospital of Southeast University
  • Shengli Liu Department of General Surgery, the Affiliated Zhongda Hospital of Southeast University
  • Xushun Liu Department of General Surgery, the 81st Hospital of P.L.A., P.L.A. Cancer Center
  • Zi Ge Department of General Surgery, the Affiliated Zhongda Hospital of Southeast University
  • Feng Wang Department of General Surgery, the 81st Hospital of P.L.A., P.L.A. Cancer Center
  • Guangquan Zong Department of General Surgery, the 81st Hospital of P.L.A., P.L.A. Cancer Center
  • Wei Wang Department of General Surgery, the 81st Hospital of P.L.A., P.L.A. Cancer Center
Keywords: P120 catenin, RT-PCR, western blotting, pancreatic carcinoma

Abstract

The aim of this work was investigate the association of P120 catenin expression with the clinicopathologic features and prognosis of pancreatic carcinoma. RT-PCR was performed to investigate the expression of P120 catenin mRNA and western blotting were performed to investigate the expression of P120 catenin protein in 52 patients with pancreatic carcinoma. The relationships between P120 catenin expression and clinicopathological characteristics and prognosis were analyzed.

The mRNA and protein expression of P120 catenin detected by RT-PCR and western blot-ting in pancreatic carcinoma was significantly lower than that in normal pancreatic tissues (0.227±0.067 vs 0.793±0.162, t=9.157, P =0.000; 0.665±0.192 vs 0.936±0.251, t=3.857=, P=0.002). Reduced expression of P120 catenin mRNA and protein was significantly correlated with lymph node metastasis (P =0.004, P =0.006), vascular invasion (P =0.022, P=0.039), distant metastasis (P =0.037, P =0.025), differentiated (P =0.033, P =0.013) and pTNM stage (P =0.003, P =0.022) of tumours. Additionally, reduced expression of P120 catenin mRNA and protein in tumour correlated with a worse prognosis and normal expression with a better survival rate (P=0.022, P=0.007). The reduced expression of both P120 catenin mRNA and protein in pancreatic carcinoma suggest that low expressions relate to pancreatic carcinoma develop- ment. P120 catenin may be related to pancreatic carcinoma behaviour and be a potential prognostic molecule.

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Expression and Clinical Significance of P120 Catenin mRNA and Protein in Pancreatic Carcinoma
Published
2009-08-20
How to Cite
1.
Fei Y, Cheng Z, Liu S, Liu X, Ge Z, Wang F, Zong G, Wang W. Expression and Clinical Significance of P120 Catenin mRNA and Protein in Pancreatic Carcinoma. Bosn J of Basic Med Sci [Internet]. 2009Aug.20 [cited 2020Sep.28];9(3):191-7. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2805
Section
Molecular Biology