Importance of Isolation and Biotypization of Gardnerella Vaginalis in Diagnosis of Bacterial Vaginosis

  • Fatima Numanović Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
  • Mirsada Hukić Institute for Microbiology, University of Sarajevo Clinics Centre
  • Mahmud Nurkić Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
  • Merima Gegić Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
  • Zineta Delibegović Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
  • Alma Imamović Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
  • Selma Pašić Polyclinic for Laboratory Diagnostics, Department for Microbiology, University Clinics Centre Tuzla
Keywords: Gardnerella vaginalis, bacterial vaginosis, biotypization

Abstract

The natural habitat of Gardnerella vaginalis is a vagina since it could be located among 69% of women who have no signs of vaginal infection and in the vagina of as many as 13,5% girls. G. vaginalis is almost certainly identified among women diagnosed with bacterial vaginosis as well as in the urethra of their sexual partner. The increase in prevalence and concentration of G. vaginalis among patients diagnosed with this syndrome confirms that G. vaginalis plays a significant role in its pathogenesis. In our research, based on Amsel criteria for three or more clinical signs of bacterial vaginosis, it was diagnosed in 20,5% of women with subjective problems of vaginal infection, and in 48,80% of women with subjective symptoms characteristic of this disease. G. vaginalis was isolated from vaginal secretion of women without clinical signs characteristic of bacterial vaginosis. In 2,58% of cases it was solitary, while in 1,28% it was found in combination with other aerobic and anaerobic bacteria and, in 1,28% women combined with Candida albicans. The isolation of G. vaginalis was significantly increased (p<0,05) in the group of women with clinical signs of bacterial vaginosis in comparison to the group of women without these signs. Frequent recurrence of bacterial vaginosis, which is found in 20-30% of women within a three months treatment, is explained as reinfection with other biotype of G. vaginalis, different from a source biotype or as a consequence of wrong treatment. Following Piot biotype scheme, biotypes 2., 3. and 7. G. vaginalis are significantly more often isolated from women who suffer from bacterial vaginosis. Biotype 7. G. vaginalis, isolated from the group of women without clinical signs of bacterial vaginosis, accounted for 2,58% cases. Following Benit biotype scheme, biotypes IVa, IVc and Ilc were identified in 12,90% cases, while biotypes Illa, IIa, Ia, IVb, IIb were found in 6,45% cases. Lipase-positive isolates of G. vaginalis were significantly more frequently accompanied by the syndrome of bacterial vaginosis.

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Published
2008-08-20
How to Cite
1.
Numanović F, Hukić M, Nurkić M, Gegić M, Delibegović Z, Imamović A, Pašić S. Importance of Isolation and Biotypization of Gardnerella Vaginalis in Diagnosis of Bacterial Vaginosis. Bosn J of Basic Med Sci [Internet]. 2008Aug.20 [cited 2020Nov.30];8(3):270-6. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2932
Section
Microbiology