Topical treatment of standardised burns with herbal remedies in model rats

  • Fahir Bečić Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine
  • Nedžad Mulabegović Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine
  • Zakira Mornjaković Institute of Histology and Embriology, Faculty of Medicine
  • Elvedina Kapić Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine
  • Senad Prašović Institute of Pathology, Faculty of Veterinary Medicine
  • Ervina Bečić Department of Analytical of Drugs, Faculty of Pharmacy
  • Jasna Kusturica Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine
Keywords: burns, animal model, rats, herbal remedies

Abstract

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

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Topical treatment of standardised burns with herbal remedies in model rats
Published
2005-11-20
How to Cite
1.
Bečić F, Mulabegović N, Mornjaković Z, Kapić E, Prašović S, Bečić E, Kusturica J. Topical treatment of standardised burns with herbal remedies in model rats. Bosn J of Basic Med Sci [Internet]. 2005Nov.20 [cited 2020Oct.23];5(4):50-7. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/3233
Section
Pharmacology