Serum extracellular vesicle microRNA dysregulation and childhood trauma in adolescents with major depressive disorder

Authors

  • Liu-yi Ran Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Yi-ting Kong Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Jiao-jiao Xiang Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Qi Zeng Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Chen-yu Zhang Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Lei Shi Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China https://orcid.org/0000-0001-8828-2724
  • Hai-tang Qiu Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Chuan Liu Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Lin-li Wu Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Ya-lan Li Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Jian-mei Chen Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Ming Ai Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
  • Wo Wang Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China
  • Li Kuang Mental Health Center, University-Town Hospital of Chongqing Medical University, Chongqing, China; Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

DOI:

https://doi.org/10.17305/bjbms.2022.7110

Keywords:

Extracellular vesicle, miRNA, childhood trauma, major depressive disorder, adolescent

Abstract

Major depressive disorder (MDD) seriously endangers adolescent mental and physical health. Extracellular vesicles (EVs) are mediators of cellular communication and are involved in many physiological brain processes. Although EV miRNAshave been implicated in adults with major psychiatric disorders, investigation into their effects in adolescent MDDremains scarce. In discovery set, we conducted a genome-wide miRNA sequencing of serum EVs from 9 untreated adolescents with MDD and 8 matched healthy controls (HCs), identifying 32 differentially expressed miRNAs (18 upregulated and 14 downregulated). In the validation set, 8 differentially expressed and highly enriched miRNAs were verified in independent samples using RT-PCR, with 4 (miR-450a-2-3p, miR-3691-5p, miR-556-3p, and miR-2115-3p) of the 8 miRNAs found to be significantly elevated in 34 untreated adolescents with MDD compared with 38 HCs and consistent with the sequencing results. After the Bonferroni correction, we found that three miRNAs (miR-450a-2-3p, miR-556-3p, and miR-2115-3p) were still significantly different. Among them, miR-450a-2-3p showed the most markeddifferential expression and was able to diagnose disease with 67.6% sensitivity and 84.2% specificity. Furthermore, miR-450a-2-3p partially mediated the associations between total childhood trauma, emotional abuse, and physical neglect and adolescent MDD. We also found that the combination of miR-450a-2-3p and emotional abuse could effectively diagnose MDD in adolescents with 82.4% sensitivity and 81.6% specificity. Our data demonstrate the association of serum EV miRNA dysregulation with MDD pathophysiology and, furthermore, show that miRNAs may mediate the relationship between early stress and MDD susceptibility. We also provide a valid integrated model for the diagnosis of adolescent MDD.

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Serum extracellular vesicle microRNA dysregulation and childhood trauma in adolescents with major depressive disorder

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Published

2022-06-03

How to Cite

1.
Ran L- yi, Kong Y- ting, Xiang J- jiao, Zeng Q, Zhang C- yu, Shi L, Qiu H- tang, Liu C, Wu L- li, Li Y- lan, Chen J- mei, Ai M, Wang W, Kuang L. Serum extracellular vesicle microRNA dysregulation and childhood trauma in adolescents with major depressive disorder. Bosn J of Basic Med Sci [Internet]. 2022Jun.3 [cited 2022Aug.18];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7110

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Section

Translational and Clinical Research