Exosome miR-4738-3p-mediated regulation of COL1A2 through the NF-κB and inflammation signaling pathway alleviates osteoarthritis low-grade inflammation symptoms

Authors

  • Jun Xu Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Kaifeng Zhou Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Huijie Gu Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Yiming Zhang Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Liang Wu Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Chong Bian Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Zhongyue Huang Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Guangnan Chen Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Xiangyang Cheng Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China
  • Xiaofan Yin Department of Orthopaedics, Minhang Hospital, Fudan University, Shanghai, China

DOI:

https://doi.org/10.17305/bb.2023.9921

Keywords:

Exosome, micro RNA (miR)-4739-3p, collagen type I alpha 2 chain (COL1A2), nuclear factor kappa B (NF-κB) signaling pathway, osteoarthritis (OA)

Abstract

This study aimed to elucidate the roles of microRNA (miR)-4738-3p and the collagen type I alpha 2 chain (COL1A2) gene in the pathogenesis of osteoarthritis (OA) through bioinformatics analysis and cellular assays. The GSE55235 dataset was analyzed using the weighted gene co-expression network analysis (WGCNA) method to identify gene modules associated with OA. Key overlapping genes were identified from these modules and the GSE55235-differential expressed genes (DEGs). The expression levels of selected genes were determined in C28/I2 cells using the quantitative real-time polymerase chain reaction (qRT-PCR). The interaction between miR-4738-3p and COL1A2 was examined in the context of interleukin 1 beta (IL-1β) induction. Exosome characterization was achieved through transmission electron microscopy (TEM), western blotting (WB), and other analyses. The study also investigated the functional relevance of miR-4738-3p in OA pathology through various molecular and cellular assays. Our findings revealed that the green module exhibited a strong correlation with the OA phenotype in the GSE55235 dataset, with COL1A2 emerging as a hub gene and miR-4738-3p as its key downstream target. IL-1β induction suggested that COL1A2 is involved in inflammation and apoptosis, while miR-4738-3p appeared to play an antagonistic role. The analysis of exosomes underscored the significance of miR-4738-3p in cellular communication, with an enhanced level of exo-miR-4738-3p antagonizing IL-1β-induced inflammation and promoting cell survival. Conversely, a reduction in exo-miR-4738-3p led to increased cell damage. This study established a clear regulatory relationship between miR-4738-3p and COL1A2, with the nuclear factor kappa B (NF-κB) signaling pathway playing a central role in this regulation. The miR-4738-3p significantly influences the OA-associated inflammation, primarily through modulation of COL1A2 and the NF-κB pathway. Therefore, targeting miR-4738-3p offers a potential therapeutic approach for OA, with exosome miR-4738-3p presenting a promising strategy.

Exosome miR-4738-3p-mediated regulation of COL1A2 through the NF-κB and inflammation signaling pathway alleviates osteoarthritis low-grade inflammation symptoms

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Published

06-12-2023

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Section

Molecular Biology

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How to Cite

1.
Exosome miR-4738-3p-mediated regulation of COL1A2 through the NF-κB and inflammation signaling pathway alleviates osteoarthritis low-grade inflammation symptoms. Biomol Biomed [Internet]. 2023 Dec. 6 [cited 2024 Feb. 24];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/9921