Tumor Suppresser Gene p53 Expression in Premalignant Lesions and Gastric Carcinoma - Prognostic Value

Zora Vukobrat-Bijedić; Svjetlana Radović; Azra Husić-Selimović; Srđan Gornjaković

doi:10.17305/bjbms.2007.3080

Authors

Zora Vukobrat-Bijedić Gastroenterohepatology Clinic, University of Sarajevo Clinics Center
Svjetlana Radović Institute of Pathology, Faculty of Medicine, University of Sarajevo
Azra Husić-Selimović Gastroenterohepatology Clinic, University of Sarajevo Clinics Center
Srđan Gornjaković Gastroenterohepatology Clinic, University of Sarajevo Clinics Center

DOI:

https://doi.org/10.17305/bjbms.2007.3080

Keywords:

intestinal metaplasia (IM), epithelial dysplasia, Helicobacter pylori (H. pylori), p53

Abstract

The aim of the study was to verify the presence of mutated tumor suppresser gene p53 in intestinal mucosa with histologically confirmed premalignant lesions and gastric carcinoma, and assess its prognostic value. The paper presents prospective study that included 50 patients with gastric adeno-carcinoma of intestinal type that were treated at Gastroenterohepatology Clinic, and 50 patients with histologically confirmed chronic atrophic H. pylori positive gastritis. In the mucosa biopsy samples, we analyzed presence, frequency and severity of inflammatory-regenerative, metaplastic and dysplastic changes. We typed intestinal metaplasia immunohistochemically and confirmed the presence of p53 onco-protein in antigen positive gastric carcinoma cells, and evaluated its prognostic value. Our results suggest that H. pylori acts as an initiator of inflammatory processes in gastric mucosa, which are followed by emergence of precancerous lesions. p53 is expressed late in carcinogenesis (14%) and as such, may be considered as an indicator of transformation of premalignant into malignant lesion.