Thin layer chromatography-application in qualitative analysis on presence of coumarins and flavonoids in plant material

  • Elvira E. Kovač-Bešović Department of Pharmacognosy, Faculty of Pharmacy; University of Sarajevo
  • Kemal Durić Department of Pharmacognosy, Faculty of Pharmacy; University of Sarajevo
Keywords: TLC, coumarins, flavonoids, dill, oats, sweet woodruff

Abstract

Drugs, natural medicinal plant, animals and mineral materials, have a large and various application in official pharmacy and medicine. Carriers of multilateral pharmacological effects that those drugs shown, are chemically define as active components that are present in them. Methods of qualitative and quantitative analysis are used for the chemical investigation of components that drugs contain. Method of thin layer chromatography has been shown as very reliable. According to the chemical investigation of single drugs, it is possible to define a group of compound or single compound comparing them with standards. Relating to the usage of method of thin layer chromatography, it has been carried out investigation on presence of coumarinsand flavonoids in domestic plant material that have wide everyday usage. Coumarins and flavonoids from the point of view of chemical belonging are phenol derivatives with important pharmacological effects. Applying method of thin layerchromatography, it is detected presence of coumarins and flavonoids substances in plant material that has been tested. Anethi graveolens fructus et folium (fruit and leaf of dill), Anethum graveolens L., Apiaceae, Avenae sativae fructus (fruit of oats), Avena sativa L., Poaceae and Asperulae odoratae herba (sweet woodruff), Asperula odorata L., Rubiaceae. Chromatograms are developed in systems cyclohexane-ethylacetat (13:7) and toluene-ether (1:1) saturated with 10% acetic acid, and visualisation by observing on UV lamp (254 and 366 nm), spraying with reagents KOH (10% ethanol solution) and diphenylboryloxyethylamine (1% methanol solution).

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Thin layer chromatography-application in qualitative analysis on presence of coumarins and flavonoids in plant material
Published
2003-08-20
How to Cite
1.
Kovač-Bešović EE, Durić K. Thin layer chromatography-application in qualitative analysis on presence of coumarins and flavonoids in plant material. Bosn J of Basic Med Sci [Internet]. 2003Aug.20 [cited 2020Oct.30];3(3):19-6. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/3523
Section
New and Emerging Methods