Inhibition of miR-9 decreases osteosarcoma cell proliferation

  • Wu Gang Department of Orthopedics, Central Theater Command General Hospital of the Chinese People's Liberation Army, Wuhan, Hubei, China
  • Wei Tanjun Department of Orthopedics, Central Theater Command General Hospital of the Chinese People's Liberation Army, Wuhan, Hubei, China
  • Huang Yong Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei, China
  • Qin Jiajun Department of Orthopedics, Central Theater Command General Hospital of the Chinese People's Liberation Army, Wuhan, Hubei, China
  • Zhang Yi Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China
  • Hu Hao Department of Orthopedics, Central Theater Command General Hospital of the Chinese People's Liberation Army, Wuhan, Hubei, China; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei, China https://orcid.org/0000-0003-4212-6704
Keywords: Osteosarcoma, cell proliferation, metastasis, miR-9, cadherin-1, CDH1, matrix metalloproteinase 13, MMP-13, forkhead box O3, FOXO3a, Bcl-2-like protein 11, BCL2L11, β-catenin, CTNNB1

Abstract

Osteosarcoma (OS) is the most common primary bone tumor that affects adolescents and young adults. Disruption of microRNA (miRNA) regulation is well established in the pathophysiology of different cancers, including OS. Increased expression of miR-9 in OS positively correlates with the tumor size, clinical stage, and distant metastasis. In the present study, we used two different OS cell lines, MG-63 and Saos-2, as in vitro models. Small interfering RNA against miR-9 and miR-9 mimics were used to study the function of miR-9 in these two cell lines. We determined the effect of miR-9 inhibition on cell proliferation, cell cycle, apoptosis, and the protein expression of different genes. Our results demonstrated that miR-9 knockdown in the human OS cell lines inhibits their metastatic potential, as determined by decreased cell proliferation and cell cycle arrest, decreased invasion, and increased apoptosis. The western blot analysis showed that cadherin-1 (CDH1), matrix metalloproteinase 13 (MMP-13), forkhead box O3 (FOXO3a), Bcl-2-like protein 11 (BCL2L11), and β-catenin (CTNNB1) are involved in miR-9 signaling. Moreover, miR-9 mimics rescued the effects caused by the inhibition of miR-9 in the OS cell lines. Our findings suggest that miR-9 is important for mediating OS cell migration, invasion, metastasis, and apoptosis. Inhibition of miR-9 could be further explored as a therapeutic target to treat OS.

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Published
2019-11-14
How to Cite
1.
Gang W, Tanjun W, Yong H, Jiajun Q, Yi Z, Hao H. Inhibition of miR-9 decreases osteosarcoma cell proliferation. Bosn J of Basic Med Sci [Internet]. 2019Nov.14 [cited 2019Dec.10];00. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/4434
Section
Molecular Biology