Increased co-expression of TIM-3 with TIGIT or 2B4 on CD8+ T cells is associated with poor prognosis in locally advanced nasopharyngeal carcinoma

Authors

  • Xiaolin Xie The Third Affiliated Teaching Hospital of Xinjiang Medical University, Affiliated Cancer Hospital, Urumqi, China; Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China https://orcid.org/0000-0002-4101-3444
  • Yaning Feng Xinjiang Key Laboratory of Oncology, Urumqi, Xinjiang, China; Key Laboratory of Oncology of Xinjiang Uyghur Autonomous Region, Urumqi, China
  • Peiwen Fan The Third Affiliated Teaching Hospital of Xinjiang Medical University, Affiliated Cancer Hospital, Urumqi, China; Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China https://orcid.org/0000-0001-5957-674X
  • Danning Dong The Third Affiliated Teaching Hospital of Xinjiang Medical University, Affiliated Cancer Hospital, Urumqi, China; Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China
  • Xuan Yao Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China; CAMS Oxford Institute (COI), University of Oxford, Oxford, United Kingdom
  • Yanchun Peng Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China; CAMS Oxford Institute (COI), University of Oxford, Oxford, United Kingdom
  • Ruozheng Wang The Third Affiliated Teaching Hospital of Xinjiang Medical University, Affiliated Cancer Hospital, Urumqi, China; Key Laboratory of Cancer Immunoterapy and Radiotherapy, Chinese Academy of Medical Sciences, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China; Xinjiang Key Laboratory of Oncology, Urumqi, Xinjiang, China; Key Laboratory of Oncology of Xinjiang Uyghur Autonomous Region, Urumqi, China

DOI:

https://doi.org/10.17305/bb.2022.8562

Keywords:

Locally advanced nasopharyngeal carcinoma, inhibitory receptor, co-expression, prognosis

Abstract

The use of immune checkpoint inhibitors in malignant tumors improves patient outcomes. Because single-agent immune checkpoint blockade has a low objective response rate, it is meaningful to explore combined blockade of immune checkpoint receptors. We aimed to investigate the co-expression of TIM-3 with TIGIT or 2B4 on peripheral blood CD8+ T cells from patients with locally advanced nasopharyngeal carcinoma. The correlation between co-expression level and clinical characteristics and prognosis was studied to provide a basis for immunotherapy for nasopharyngeal carcinoma. Flow cytometry was used to detect TIM-3/TIGIT and TIM-3/2B4 co-expression on CD8+ T cells. The differences in co-expression between patients and healthy controls were analyzed. The correlation between co-expression of TIM-3/TIGIT or TIM-3/2B4 and the patient clinical characteristics and prognosis was examined. Also, the correlation between the TIM-3/TIGIT or 2B4 co-expression and other common inhibitory receptors was analyzed. We further validated our results using mRNA data from the Gene Expression Omnibus (GEO) database. TIM-3/TIGIT and TIM-3/2B4 co-expression was upregulated on peripheral blood CD8+ T cells from patients with nasopharyngeal carcinoma. They were both correlated with poor prognosis. There was a correlation between TIM-3/TIGIT co-expression and patient age and pathological stage, whereas TIM-3/2B4 co-expression correlated with age and sex. CD8+ T cells with elevated mRNA levels of TIM3/TIGIT and TIM3/2B4 also showed increased expression of multiple inhibitory receptors, indicating T cell exhaustion in locally advanced nasopharyngeal carcinoma. TIM-3/TIGIT or TIM-3/2B4 can be used as potential targets for combination immunotherapy in locally advanced nasopharyngeal carcinoma.

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Increased co-expression of TIM-3 with TIGIT or 2B4 on CD8+ T cells is associated with poor prognosis in locally advanced nasopharyngeal carcinoma

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Published

03-07-2023

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Section

Immunology

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How to Cite

1.
Increased co-expression of TIM-3 with TIGIT or 2B4 on CD8+ T cells is associated with poor prognosis in locally advanced nasopharyngeal carcinoma. Biomol Biomed [Internet]. 2023 Jul. 3 [cited 2024 May 19];23(4):584–595. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/8562