Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence

Authors

  • Mohd Adnan Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia https://orcid.org/0000-0002-7080-6822
  • Arif Jamal Siddiqui Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia https://orcid.org/0000-0002-6236-0920
  • Emira Noumi Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia
  • Syed Amir Ashraf Department of Clinical Nutrition, College of Applied Medial Sciences, University of Ha’il, Ha’il, Saudi Arabia https://orcid.org/0000-0002-5333-9505
  • Amir Mahgoub Awadelkareem Department of Clinical Nutrition, College of Applied Medial Sciences, University of Ha’il, Ha’il, Saudi Arabia
  • Sibte Hadi Department of Forensic Sciences, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia https://orcid.org/0000-0002-2994-3083
  • Mejdi Snoussi Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia
  • Riadh Badraoui Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia https://orcid.org/0000-0001-9054-7744
  • Fevzi Bardakci Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia https://orcid.org/0000-0003-3377-2763
  • Manojkumar Sachidanandan Department of Oral Radiology, College of Dentistry, University of Ha’il, Ha’il, Saudi Arabia
  • Mitesh Patel Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara, India https://orcid.org/0000-0002-9283-2124

DOI:

https://doi.org/10.17305/bb.2023.9324

Keywords:

Biosurfactant, Lactobacillus acidophilus, antibiofilm, quorum sensing (QS), virulence

Abstract

Antimicrobial resistance by pathogenic bacteria has become a global risk to human health in recent years. The most promising approach to combating antimicrobial resistance is to target virulent traits of bacteria. In the present study, a biosurfactant derived from the probiotic strain Lactobacillus acidophilus was tested against three Gram-negative bacteria to evaluate its inhibitory potential on their biofilms, and whether it affected the virulence factors controlled by quorum sensing (QS). A reduction in the virulence factors of Chromobacterium violaceum (violacein production), Serratia marcescens (prodigiosin production) and Pseudomonas aeruginosa (pyocyanin, total protease, LasB elastase and LasA protease production) was observed at different sub-MIC concentrations in a dose-dependent manner. Biofilm development was reduced by 65.76%, 70.64% and 58.12% at the highest sub-MIC levels for C. violaceum, P. aeruginosa and S. marcescens, respectively. Biofilm formation on glass surfaces exhibited significant reduction, with less bacterial aggregation and reduced formation of extracellular polymeric materials. Additionally, swimming motility and exopolysaccharides (EPS) production were shown to be reduced in the presence of the L. acidophilus-derived biosurfactant. Furthermore, molecular docking analysis performed on compounds identified through gas chromatography–mass spectrometry (GC-MS) analysis of QS and biofilm proteins yielded further insights into the mechanism underlying the anti-QS activity. Therefore, the present study has clearly demonstrated that a biosurfactant derived from L. acidophilus can significantly inhibit virulence factors of Gram-negative pathogenic bacteria. This could provide an effective method to inhibit the formation of biofilms and QS in Gram-negative bacteria.

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Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence

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Published

03-11-2023

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Section

Microbiology

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How to Cite

1.
Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence. Biomol Biomed [Internet]. 2023 Nov. 3 [cited 2024 May 19];23(6):1051–1068. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/9324