Direct Immunofluorescence and Immunohistochemistry in Diagnostics of Glomerulonephritis

  • Zinaida Karasalihović Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla
  • Ermina Iljazović Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla
  • Dušan Ferluga Institute of Pathology, Faculty of Medicine, University of Ljubljana
  • Elmir Čičkušić Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla
  • Jasminka Mustedanagić Mujanović Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla
  • Jugoslav Stahov Faculty of Natural Sciences and Mathematics, University of Tuzla
  • Ina Škaljić Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla
Keywords: immunofluorescence (IF), immunoperoxidase (IP), renal biopsies, immune deposits

Abstract

The needle biopsies from 60 transplanted and native kidneys have been processed and a prospective analysis of pattern, intensity and distribution of immunoglobulin deposits (IgA, IgG and IgM) and complement components (C3c and C1q) identified in these lesions has been carried out by immunohistochemistry with three step immunoperoxidase, in the period from 2000 to 2004. Those deposits were previously detected and analyzed by immunofluorescence. The samples consisted of 30 renal biopsies, previously diagnosed with glomerulonephritis and positive immunofluorescence and 30 renal biopsies without morphologic changes and deposits on immunofluorescence. 78,7% of the analyzed samples showed the identical results of the deposits of immunoglobulin and components of the complement with both, immunohistochemistry and immunofluorescence method. Sensitivity of the immunohistochemistry method with three step immunoperoxidase for all analyzed immunoglobulin and complement components is high (0,93), while specificity for the same method is 0,79. Standardized method of the three step immunoperoxidase on the paraffin embedded, formalin fixed needle renal biopsies could successfully replace the immunofluorescence method in diagnostic of GN, with the emphasis on a follow up and control of each single step in the procedure of the method.

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Direct Immunofluorescence and Immunohistochemistry in Diagnostics of Glomerulonephritis
Published
2008-02-20
How to Cite
1.
Karasalihović Z, Iljazović E, Ferluga D, Čičkušić E, Mustedanagić MujanovićJ, Stahov J, Škaljić I. Direct Immunofluorescence and Immunohistochemistry in Diagnostics of Glomerulonephritis. Bosn J of Basic Med Sci [Internet]. 2008Feb.20 [cited 2020Dec.1];8(1):12-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2989
Section
Immunology