The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats

  • Nesina Avdagić Institute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo
  • Emina Nakaš-Ićindić Institute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo
  • Senija Rašić Institute of Nephrology, University Clinical Centre Sarajevo
  • Almira Hadžović-Džuvo Institute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo
  • Asija Začiragić Institute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo
  • Amina Valjevac Institute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo
Keywords: nitric oxide, inducible nitric oxide synthase, gentamicin, acute tubular necrosis

Abstract

The aim of this study was to investigate the role of inducible nitric oxide synthase (iNOS) in gentamicin-induced acute tubular necrosis in rats using the iNOS inhibitor L-N6-(1-iminoethyl) lysine (L-NIL). Wistar rats, both sexes (n=18), were equally divided into three groups. Gentamicin group received intraperitoneally (i.p.) gentamicin in 0,9 % NaCl at a dose of 80 mg/kg/day for five consecutive days. L-NIL+gentamicin group received L-NIL at a dose of 3mg/kg i.p. 36, 24 and 12 h before first dose of gentamicin. Control group received 0,9 % NaCl i.p. for five consecutive days at the equal volume as gentamicin group. Griess reaction was used for determination plasma level of NO. Semiquantitative histological analysis was used for the evaluation of kidney damage level. The plasma NO level and the level of kidney damage were statistically higher in gentamicin group in comparison to the control group (p=0,046). Application of L-NIL prior to gentamicin led to certain decrease in the plasma level of NO as well as in the level of kidney damage. Application of L-NIL, prior to gentamicin administration, did not provide complete protective effects of L-NIL on the kidney, which was demonstrated on kidney sections. The lack of anticipated protective effect of L-NIL on kidney tissue might be explained with the fact that we have used L-NIL prior but not during/after gentamicin administration. It would be necessary to examine the effects of L-NIL administration not only before, but as well during and possibly after the administration of gentamicin.

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The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats
Published
2007-11-20
How to Cite
1.
Avdagić N, Nakaš-IćindićE, Rašić S, Hadžović-DžuvoA, Začiragić A, Valjevac A. The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats. Bosn J of Basic Med Sci [Internet]. 2007Nov.20 [cited 2020Dec.2];7(4):345-51. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/3025
Section
Biochemistry