Long noncoding RNA FAM225B facilitates proliferation and metastasis of nasopharyngeal carcinoma cells by regulating miR-613/CCND2 axis

  • Weijun Dai Department of Otolaryngology, Shanghai Pudong New Area Gongli Hospital,Shanghai, China
  • Yi Shi Department of Otolaryngology, Central Hospital Minhang District, Shanghai, China
  • Weiqi Hu Department of Otolaryngology, Central Hospital Minhang District, Shanghai, China
  • Chenjie Xu Department of Otolaryngology Head and Neck Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Keywords: Nasopharyngeal carcinoma, lncRNA, FAM225B, metastasis, CCND2

Abstract

Growing evidence has suggested that abnormally expressed long non-coding RNAs (lncRNAs) play critical regulatory roles in nasopharyngeal carcinoma (NPC) pathogenesis. Family with sequence similarity 225 member B (FAM225B) is a novel lncRNA that has been implicated in several human cancers, yet its role in the context of NPC remains largely unclear. The aim of this study was to determine the expression level of FAM225B and its clinical significance in NPC patients. We observed a remarkable increase of FAM225B in NPC tissues and cell lines compared with controls. Also, highly expressed FAM225B was closely correlated with advanced TNM stage, distant metastasis, and poor overall survival. Interestingly, loss-of-function analysis revealed that FAM225B knockdown significantly inhibited tumor growth in vitro and in vivo, and decreased the migratory and invasive capacity of NPC cells. Mechanically, FAM225B functioned as an endogenous sponge by competing for miR-613 binding to up-regulate CCND2 expression. More importantly, rescue experiments further demonstrated that the suppressive impacts of FAM225B knockdown on cell proliferation, migration and invasion were significantly reversed after CCND2 overexpression. Taken all together, these findings highlight FAM225B as an oncogene that promotes NPC proliferation and metastasis through miR-613/CCND2 axis.

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Long noncoding RNA FAM225B facilitates proliferation and metastasis of nasopharyngeal carcinoma cells by regulating miR-613/CCND2 axis
Published
2021-06-17
How to Cite
1.
Dai W, Shi Y, Hu W, Xu C. Long noncoding RNA FAM225B facilitates proliferation and metastasis of nasopharyngeal carcinoma cells by regulating miR-613/CCND2 axis. Bosn J of Basic Med Sci [Internet]. 2021Jun.17 [cited 2021Sep.29];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/5691
Section
Molecular Biology